MEMBERS

ROXANA NOEMÍ PERONI

Independent Researcher - Scientific and Technological Researcher Program at CONICET. Also, Head of Practical Work - Chair of Pharmacology, Department of Pharmacology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires.

ANDREINA QUEVEDO

PhD fellow, CONICET.

Pharmacist and Biochemist- Universidad de Buenos Aires.Assistant - Chair of Analytical Instrumental Chemistry, Department of Analytical Chemistry and Physicochemistry, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires.

NATALIA POZNANSKI 

PhD fellow, UBA.

 

LINES OF RESEARCH

  1. SEARCH FOR INHIBITORS OF ABC TRANSPORTER PROTEINS IN NATURAL PRODUCTS: POTENTIAL USE AS RESENSITIZING AGENTS IN CHEMOTHERAPY

This research focuses on finding new, potent, selective, and safe inhibitors of ABC transporters that affect drug bioavailability and are linked to drug resistance in cancer and other diseases. We have observed that consuming arugula leaves (Eruca vesicaria and Diplotaxis tenuifolia varieties) and extracts of Cannabis sativa have anti-proliferative effects and can inhibit the activity of ABC transporters involved in drug resistance. Our goal is to further study the interaction of these natural compounds with ABC transporters at the molecular level using in vitro methods and human tumor cells from liver carcinoma, colorectal carcinoma, and breast cancer. The aim is to validate a biomethod for screening the activity of compounds isolated from plant material to develop new, more effective therapeutic targets for cancer treatment.

 

  1. PARTICIPATION OF OATP4C1 AND MRP4 TRANSPORTERS IN THE PROGRESSION OF AUTOSOMAL DOMINANT POLYCYSTOX KIDNEY DISEASE (ADPKD) AND ITS CORRELATION WITH URINARY cAMP LEVELS: SEARCH FOR EARLY BIOMARKERS OF PROGRESSION

This research involves a clinical study to determine if monitoring free urinary cAMP or in extracellular vesicles (EVs), as well as the renal transporters MRP4 and OATP4C1, in urinary sediment or EVs, allows for early prediction of chronic kidney failure in ADPKD patients. The functionality of renal MRP4 and OATP4C1 at the in vitro level in cell cultures will also be evaluated to ascertain whether they could be potential therapeutic targets.

 

  1. DEVELOPMENT AND VALIDATION OF A COST-EFFECTIVE MAGNETIC METHOD FOR ISOLATING EXTRACELLULAR VESICLES FOR APPLICATIONS IN PROLIFERATIVE NEPHROPATHOLOGIES AT THE CLINICAL LEVEL

This research aims to develop a cost-effective method for isolating extracellular vesicles called exosomes, which are important in cell communication and as biomarkers for diseases. Standard methods for isolating exosomes involve ultracentrifugation, which may not be feasible in clinical laboratories. This study aims to address these challenges and provide a more practical method for isolating exosomes.

 

PUBLICATIONS 

 

  1. Rosenberg ML, Yaneff A, Ferradás GM, Villafañe-Tapia MP, Davio CA, Goette NP, Vlachovsky SG, Peroni RN, Oddo EM, Azurmendi PJ. Urinary Total and Extracellular Vesicles cAMP Content Are Associated with Autosomal Dominant Polycystic Kidney Disease (ADPKD) Progression. Life 2023, 13, 1817. https://doi.org/10.3390/life13091817.
  2. Quijia CR, Quevedo-Enríquez SA, Zappia CD, Peroni RN, Chorilli M. Administration of inhibitory molecules in nanoparticles in breast cancer therapy. Current Medicinal Chemistry journal. Curr Med Chem. 2023 doi: 10.2174/0929867330666230608145125. PMID: 37291791.
  3. Minoia JM, Filia MF, Roma MI, De Fino FT, Copello GJ, Peroni RN. Selective modulation of placental and fetal MDR transporters by chronic in utero exposure to NRTIs in Sprague-Dawley rats: importance for fetoprotection. Toxicol Appl Pharmacol. 2022, 450, 116170.
  4. Roma MI, Schiariti Lampropulos VE, Ayllón-Cabrera I, Salazar Sanabria AN, López Nigro MM, Peroni RN*, Carballo MA. Modulation of hepatic ABC transporters by Eruca vesicaria intake: Potential diet-drug interactions. Food and Chemical Toxicology 2019, 133 *última autoría compartida
  5. López Nigro MM, Peroni RN*, Ayllón-Cabrera I, Schiariti Lampropulos VE, Roma MI, Carballo MA. In vivo antigenotoxic activity of Diplotaxis tenuifolia against cyclophosphamide-induced DNA damage: Relevance of modulation of hepatic ABC efflux transporters. Mutat Res Genet Toxicol Environ Mutagen. 2018, 836(Pt B):72-78. *primera autoría compartida
  6. Andrea M. Mebert, Gisela S. Alvarez, Roxana Peroni, Corinne Illoul, Christophe Hélary, Thibaud Coradin, Martin F. Desimone. Collagen-silica nanocomposites as dermal dressings preventing infection in vivo. Materials Science & Engineering C. 2018, 93:170-177.
  7. Gantner ME, Peroni RN, Morales JF, Villalba ML, Ruiz ME, Talevi A. Development and Validation of a Computational Model Ensemble for the Early Detection of BCRP/ABCG2 Substrates during the Drug Design Stage. J Chem Inf Model. 2017 (8):1868-1880.
  8. Filia MF, Marchini T, Minoia JM, Roma MI, De Fino FT, Rubio MC, Copello GJ, Evelson PA, Peroni RN. Induction of ABCG2/BCRP restricts the distribution of zidovudine to the fetal brain in rats. Toxicol Appl Pharmacol. 2017; 330:74-83.
  9. Carballo MA., Roma MI, Ayllón-Cabrera I, López Nigro MM, Peroni RN. "Chronic intake of wild rocket leaf juice-. Antigenotoxic effect and modulation of hepatic ABC efflux transporters". Abstracts/Toxicology Letters 2595, 2016 S73-S247
  10. Roma MI, Hocht C, Chiappetta DA, Di Gennaro SS, Minoia JM, Bramuglia GF, Rubio MC, Sosnik A, Peroni RN. Tetronic® 904-containing polymeric micelles overcome the overexpression of ABCG2 in the blood-brain barrier of rats and boost the penetration of the antiretroviral efavirenz into the CNS. Nanomedicine (Lond). 2015 (15):2325-37.
  11. Peroni RN, Abramoff T, Neuman I, Podestá EJ, Adler-Graschinsky E. Phytoestrogens enhance the vascular actions of the endocannabinoid anandamide in mesenteric beds of female rats. Int J Hypertens. 2012; 2012:647856.
  12. Peroni, RN. Influencia de la proteína de resistencia al cáncer de mama (BCRP) en la farmacocinética de los antirretrovirales. Rev. Farm. vol. 153 nº 1-2: 91-102, 2011.
  13. Peroni RN, Di Gennaro SS, Hocht C, Chiappetta DA, Rubio MC, Sosnik A, Bramuglia GF. Efavirenz is a substrate and in turn modulates the expression of the efflux transporter ABCG2/BCRP in the gastrointestinal tract of the rat. 2011, 82(9):1227-33.
  14. Orliac ML, Peroni RN, Abramoff T, Neuman I, Podesta EJ, Adler-Graschinsky E. Increases in vanilloid TRPV1 receptor protein and CGRP content during endotoxemia in rats. Eur J Pharmacol. 2007 ;566 (1-3):145-52.
  15. Peroni RN, Orliac ML, Abramoff T, Ribeiro ML, Franchi AM, Adler-Graschinsky E. Participation of CGRP and prostanoids in the sex-linked differences of vascular anandamide effects in mesenteric beds of Sprague-Dawley rats. Eur J Pharmacol. 2007, 557(1):49-57. ISSN:00142999 FI2007: 2,853
  16. Peroni RN, Orliac ML, Becu-Villalobos D, Huidobro-Toro JP, Adler-GraschinskyE, Celuch SM. Sex-linked differences in the vasorelaxant effects of anandamide invascular mesenteric beds: role of oestrogens. Eur J Pharmacol. 2004;493(1-3):151-60.
  17. Orliac ML, Peroni R, Celuch SM, Adler-Graschinsky E. Potentiation of anandamide effects in mesenteric beds isolated from endotoxemic rats. J Pharmacol Exp Ther. 2003, 304(1):179-84.

 

BOOK CHAPTERS

“Discovering new antiepileptic drugs addressing the transporter hypothesis of refractory epilepsy. Ligand-based approximations”, by Couyupetrou M, Di Ianni ME, Gantner ME, Pesce G, Peroni R, Bruno-Blanch LE & Talevi A, 2012. Antiepileptic Drug Discovery Novel Approaches.

 

FUNDING SOURCES

NATIONAL

  1. PICT 2021-I-0155 “Development and validation of a cost-effective and reproducible magnetic method for the isolation of urinary exosomes for applications in proliferative renal pathologies at the clinical level”. Director: Roxana Peroni
  2. National Cancer Institute, Ministry of Health DI-2020-85013845-APN-INC#MS. “Development of magnetic nanoparticles for the treatment of breast tumors resistant to conventional therapies. Director: Alejandro Berra, Co-director: Roberto Zysler. Participating researcher: Roxana Peroni
  3. PICT 2018-D-02279 “Clinical-molecular study of the participation of OATP41C and MRP4 transporters in the progression of autosomal dominant polycystic kidney disease and its correlation with urinary cAMP concentrations: Rational search for new therapeutic targets”. Director: Roxana Peroni

INTERNATIONAL

  • NESTOR Project, "Nanomaterials for Enzymatic Control of Oxidative Stress Toxicity and Free Radical Generation"; 101007629 2020 – Marie Skłodowska-Curie Research and Innovation Staff Exchange (MSCA-RISE), European Union. https://nestor.unizar.es/

 

COLLABORATIONS

INTERNATIONAL

  • Dr. Gerardo Goya. Institute of Nanoscience and Materials of Aragon, University of Zaragoza, Spain.
  • Dr. Bojana Segura, National Institute of Biology, Ljubljana, Slovenia.
  • ​​Dr. Vittoria Raffa, Department of Biology, University of Pisa, Italy.

 

NATIONAL

  • Dr. Roberto Zysler and team. Institute of Nanoscience and Nanotechnology - INN-CNEA Bariloche-CONICET
  • Dr. Pablo Azurmendi and team. Experimental Nephrology and Molecular Biochemistry Service. Alfredo Lanari Medical Research Institute- UBA-CONICET
  • Dr. Marta Carballo and team. INFIBIOC-Institute of Physiopathology and Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires.
  • Dr. Ana Vanesa Torbidoni. Molecular Biology Laboratory (BioCyM), Argentine Institute of Veterinary Medicine, Environment and Health (IAVAS), Juan Agustín Maza University.